Recombinant Human Proteasome subunit beta type 4 His Protein

Name: Recombinant Human Proteasome subunit beta type 4 His Protein
Brand: Novus Biologicals
SKU: NBP1-72545

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72545

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Proteasome subunit beta type 4 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 46-264 of Human Proteasome subunit beta type 4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTQNPMVTGT SVLGVKFEGG VVIAADMLGS YGSLARFRNI SRIMRVNNST MLGASGDYAD FQYLKQVLGQ MVIDEELLGD GHSYSPRAIH SWLTRAMYSR RSKMNPLWNT MVIGGYADGE SFLGYVDMLG VAYEAPSLAT GYGAYLAQPL LREVLEKQPV LSQTEARDLV ERCMRVLYYR DARSYNRFQI ATVTEKGVEI EGPLSTETNW DIAHMISGFE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

26.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Proteasome subunit beta type 4 His Protein

SDS-PAGE: Recombinant Human Proteasome subunit beta type 4 His Protein [NBP1-72545]

SDS-Page: Recombinant Human Proteasome subunit beta type 4 Protein [NBP1-72545] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72545

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome subunit beta type 4

Proteasome subunit beta type-4, also known as PSMB4, belonged to the peptidase T1B family. The 20S Proteasome chamber contains alpha subunits (which are structural) and beta subunits (which are predominantly catalytic). The outer two rings in the proteasome consist of seven alpha subunits each and the inner two rings each consist of seven beta subunits. PSMB4 is a beta subunit of the 20S Proteasome. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMB4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Proteasome subunit beta type-4

Alternate Names

HsBPROS26, HsN3, PROS-26, PROS26, Proteasome chain 3, PSMB4

Gene Symbol

PSMB4

Additional Proteasome subunit beta type 4 Products

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COA

Product Specific Notices for Recombinant Human Proteasome subunit beta type 4 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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