Protein A Native Protein

Name: Protein A Native Protein
Brand: Novus Biologicals
SKU: NBP1-97288

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97288

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Accession #

P38507

Conjugate

Sepharose

Applications

Immunoprecipitation

View all Protein A Proteins and Enzymes »

Product Specifications

Description

Native Protein A (Accession #: P38507))

Store SEPHAROSE PROTEIN A at 4C prior to opening. DO NOT FREEZE.

Purity

N/A

Application Notes

Protein A resin may also be used to pull down antibody:antigen complexes in immunoprecipitation experiments. This product is suitable for use at 20 uL per immunoprecipitation reaction.

Protein / Peptide Type

Native Protein

Scientific Data Images for Protein A Native Protein

Immunoprecipitation: Protein A Native Protein [NBP1-97288]

Immunoprecipitation: Protein A Native Protein [NBP1-97288] - Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding.

Formulation, Preparation and Storage

NBP1-97288

Preparation Method	Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2, and rabbit. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants/dilute cell cultures. High capacity and high flow rate fractionation is applicable to both laboratory and scale-up processes. Sepharose(TM) Protein A can be used for immunoprecipitation and purification of monoclonal antibodies.
Formulation	20% (v/v) Ethanol
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C. Do not freeze.

Background: Protein A

Sepharose Protein A is a suspension of beads conjugated to native protein A derived from Staphylococcus aureaus. When evenly dispersed approximately 10 mL of suspension is equal to 5.0 cc of settled beads of Sepharose 4 Fast Flow (highly cross linked, 4% agarose derivative). The bead size is approximately 45-165 um when swollen. Approximately 6.0 mg of Protein A is bound per cc of drained beads. Each cc of drained beads will bind approximately 35 mg Human IgG. The exclusion limit (Mr) of the beads is 3 x 10^7. The maximum linear flow rate is approximately 1300 cm/hr. This product shows long term stability within pH range of 3 to 9 and short term stability within pH range of 2 to10.

Alternate Names

PROA, Protein-A

Gene Symbol

SAOUHSC_00069

UniProt

P38507 (Bacteria)

Additional Protein A Products

Product Documents for Protein A Native Protein

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COA

Product Specific Notices for Protein A Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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