Recombinant Protein A Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34964
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, HPLC, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 296 amino acids corresponding to Protein A Source: E. coli
Uniprot ID: P02976
Amino Acid Sequence: NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEED
This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Protein A as determined by LAL method.
Predicted Molecular Mass
33.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses. It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
Specificity
Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Protein A Protein
![SDS-PAGE: Recombinant Protein A Protein [NBP2-34964]](https://resources.bio-techne.com/images/products/Protein-A-Protein-SDS-Page-NBP2-34964-img0001.jpg "SDS-PAGE: Recombinant Protein A Protein [NBP2-34964]")
SDS-PAGE: Recombinant Protein A Protein [NBP2-34964]
SDS-Page: Protein A Protein [NBP2-34964]Formulation, Preparation and Storage
NBP2-34964
| Formulation | Lyophilized from additive free solution. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Dissolve in distilled water or saline. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Protein A
Alternate Names
PROA, Protein-A
Gene Symbol
SAOUHSC_00069
Additional Protein A Products
Product Documents for Recombinant Protein A Protein
Product Specific Notices for Recombinant Protein A Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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