Recombinant Human PSMB7 His Protein

Name: Recombinant Human PSMB7 His Protein
Brand: Novus Biologicals
SKU: NBP1-99093

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99093

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all PSMB7 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 44-277 of Human Proteasome 20S beta 7

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTTIAGVVYK DGIVLGADTR ATEGMVVADK NCSKIHFISP NIYCCGAGTA ADTDMTTQLI SSNLELHSLS TGRLPRVVTA NRMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGST DKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI AAGIFNDLGS GSNIDLCVIS KNKLDFLRPY TVPNKKGTRL GRYRCEKGTT AVLTEKITPL EIEVLEETVQ TMDTS

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

TMW confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PSMB7 His Protein

SDS-PAGE: Recombinant Human PSMB7 His Protein [NBP1-99093]

SDS-Page: Recombinant Human PSMB7 His Protein [NBP1-99093] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-99093

Formulation	20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMB7

PSMB7, also known as proteasome subunit beta type-7, is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i. Recombinant human PSMB7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 7

Alternate Names

Macropain Chain Z, Proteasome Subunit Z

Gene Symbol

PSMB7

Additional PSMB7 Products

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COA

Product Specific Notices for Recombinant Human PSMB7 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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