Recombinant Human Rad1 His Protein

Name: Recombinant Human Rad1 His Protein
Brand: Novus Biologicals
SKU: NBP1-45298

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45298

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Rad1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-282 of Human Rad1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

33.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Rad1 His Protein

SDS-Page: Recombinant Human Rad1 Protein [NBP1-45298]

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-45298

Formulation	20 mM Tris-HCl buffer (pH8.0), 0.2 M Nacl, 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad1

RAD1 is a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. This complex also contains the Rad9 and Hus1 proteins and is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Recombinant human RAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

cell cycle checkpoint protein Hrad1, checkpoint control protein HRAD1, DNA repair exonuclease rad1 homolog, DNA repair exonuclease REC1, EC 3.1.11.2, exonuclease homolog RAD1, HRAD1, RAD1 (S. pombe) homolog, RAD1 homolog (S. pombe), Rad1-like DNA damage checkpoint protein, REC1cell cycle checkpoint protein RAD1

Gene Symbol

RAD1

Additional Rad1 Products

Product Documents for Recombinant Human Rad1 His Protein

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COA

Product Specific Notices for Recombinant Human Rad1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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