Recombinant Human RAP1A His Protein

Name: Recombinant Human RAP1A His Protein
Brand: Novus Biologicals
SKU: NBP1-45263

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45263

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Rap1A Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-181 of Human RAP1A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RAP1A His Protein

SDS-PAGE: Recombinant Human RAP1A His Protein [NBP1-45263]

SDS-Page: Recombinant Human RAP1A Protein [NBP1-45263] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-45263

Formulation	20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 5mM DTT, 200 mM NaCl
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rap1A

Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Recombinant human RAP1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Ras-related Protein 1A

Alternate Names

KREV-1, SMGP21

Gene Symbol

RAP1A

Additional Rap1A Products

Product Documents for Recombinant Human RAP1A His Protein

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COA

Product Specific Notices for Recombinant Human RAP1A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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