RAP80 Antibody Blocking Peptide

Name: RAP80 Antibody Blocking Peptide
Brand: Novus Biologicals
SKU: NBP1-76829PEP

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76829PEP

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Synthetic

Conjugate

Unconjugated

Applications

Antibody Competition

View all RAP80 Proteins and Enzymes »

Product Specifications

Description

A blocking peptide containing 17 amino acids near the amino terminus of human RAP80.

Source: Synthetic

(Accession #: EAW85043)

Purity

N/A

Application Notes

This peptide is useful as a blocking peptide for NBP1-76829. For further blocking peptide related protocol, click here.

Protein / Peptide Type

Antibody Blocking Peptide

Scientific Data Images for RAP80 Antibody Blocking Peptide

Western Blot: RAP80 Antibody Blocking Peptide [NBP1-76829PEP]

Western Blot: RAP80 Peptide [NBP1-76829PEP] - blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37C.

Formulation, Preparation and Storage

NBP1-76829PEP

Formulation	PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin.
Preservative	0.02% Sodium Azide
Concentration	0.2 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: RAP80

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. TRAP80 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. TRAP80 is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Long Name

Receptor Associated Protein 80

Alternate Names

RXRIP110, UIMC1, X2HRIP110

Gene Symbol

UIMC1

Additional RAP80 Products

Product Documents for RAP80 Antibody Blocking Peptide

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Product Specific Notices for RAP80 Antibody Blocking Peptide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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