Recombinant C. difficile Toxin A/TcdA Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8619-GT

R&D Systems, part of Bio-Techne
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8619-GT-020
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Key Product Details

Source

E. coli

Accession #

P25738 (msyB) & P16154 (TcdA)

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Toxin A/TcdA Proteins and Enzymes »

Product Specifications

Source

E. coli-derived c. difficile Toxin A/TcdA protein
msyB
(Thr2-Arg124)
Accession # P25738		 GSENLYFQGH C. difficile TcdA
(Met1-Leu542, Arg184Asn)
Accession # P16154		 HHHHHH
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr2 (msyB)

Predicted Molecular Mass

79 kDa

SDS-PAGE

78 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze UDP-Glucose.
The specific activity is >15 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8619-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Toxin A/TcdA

Clostridium difficile is the leading cause of hospital-acquired diarrhea, known as C. difficile-associated disease (1, 2). The major virulence factors produced by C. difficile are two toxins, TcdA and TcdB. Both toxins can monoglucosylate and inactivate Rho family small GTPases within target cells, leading to disruption of vital signaling pathways in the cell, subsequently causing diarrhea, inflammation, and damage of colonic mucosa (3  4, 5). Both toxins have a similar tripartite structure comprised of an N-terminal glucosyltransferase domain, a C-terminal receptor binding domain, and a small hydrophobic span possibly involved in toxin translocation (6). This recombinant TcdA only contains the enzymatic domain. Both TcdA and TcdB also have potassium-dependent UDP-Glc hydrolase activity, which is essentially glucosyltransferase activity with water as the acceptor molecule (7). Under same conditions, UDP-glucose hydrolysis by TcdB occurs at a rate about
5-fold greater than that of TcdA.

References

  1. Wilkins, T.D. and Lyerly, D.M. (2003) J. Clin. Microbiol 41:53.
  2. Kyne, L. et al. (2002) Clin. Infect. Dis. 34:346.
  3. Voth, D.E. and Ballard, J.D. (2005) Clin. Microbiol. Rev. 18:247.
  4. Chaves-Olarte, E. et al. (1996) J. Biol. Chem. 271:6925.
  5. Just I, et al. (1995) J. Biol. Chem. 270:13932.
  6. Hammond, G.A. and Johnson, J.L. (1995) Microb. Pathog. 19:203.
  7. Ciesla, W.P. Jr. and Bobak, D.A. (1998) J. Biol. Chem. 273:16021.

Alternate Names

ToxA, Toxin A

Entrez Gene IDs

8470358 (C. difficile)

Gene Symbol

TCDA

UniProt

P25738 (msyB) & P16154 (TcdA)

Additional Toxin A/TcdA Products

Product Documents for Recombinant C. difficile Toxin A/TcdA Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant C. difficile Toxin A/TcdA Protein, CF

For research use only

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