Recombinant Canine CD4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9646-CD

R&D Systems, part of Bio-Techne
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9646-CD-050
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Key Product Details

Source

NS0

Accession #

P33705

Conjugate

Unconjugated

View all CD4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived canine CD4 protein
Val25-Lys401, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val25

Predicted Molecular Mass

43 kDa

SDS-PAGE

42-61 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9646-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes (1). CD4 is a standard phenotype marker for the identification of T cell populations (1). CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes (2, 3). CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV (4). Mature canine CD4 consists of a 377 aa extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the extracellular region, canine CD4 shares 57%, 46%, and 42% amino acid sequence identity with human, mouse, and rat CD4, respectively.

References

  1. Vignali, D.A.A. (2010) J. Immunol. 184:5933.
  2. Doyle, C. and J.L. Strominger (1987) Nature 330:256.
  3. Huppa, J.B. et al. (2010) Nature 463:963.
  4. Biswas, P. et al. (2003) Blood 101:4452.
  5. Maddon, P.J. et al. (1985) Cell 42:93.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

UniProt

P33705

Additional CD4 Products

Product Documents for Recombinant Canine CD4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Canine CD4 Protein, CF

For research use only

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