Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein
R&D Systems, part of Bio-Techne | Catalog # 579-R2
Key Product Details
Product Specifications
Source
E. coli-derived cotton rat IL-2 protein
Ala21-Gln155 (Cys146Tyr), with and without an N-terminal Met
Ala21-Gln155 (Cys146Tyr), with and without an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met & Ala21
Predicted Molecular Mass
15.6 kDa
Activity
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.
The ED50 for this effect is 0.15-0.6 ng/mL.
The ED50 for this effect is 0.15-0.6 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 579-R2
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 579-R2/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-2
Interleukin 2 (IL-2) is a protein that has a variety of immunological functions, the most notable being the ability of IL-2 to promote the proliferation and maturation of activated T cells. Other biological activities attributed to IL-2 include:
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
UniProt
Additional IL-2 Products
Product Documents for Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein
Product Specific Notices for Recombinant Cotton Rat IL-2 (Cys146Tyr) Protein
For research use only
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