Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10108-B7

R&D Systems, part of Bio-Techne
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10108-B7-050
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Key Product Details

Source

HEK293

Accession #

XP_005548285

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all B7-H7/HHLA2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey B7-H7/HHLA2 protein
Cynomolgus Monkey
B7-H7
(Ile21-Asn345)
Accession # XP_005548285		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile21

Predicted Molecular Mass

64 kDa

SDS-PAGE

92-109 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein, CF

Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein Inhibition Activity

Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein Inhibition Activity

Recombinant Cynomolgus Monkey B7-H7/HHLA2 Fc Chimera (Catalog # 10108-B7) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-6 μg/mL.
Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein SDS-PAGE

1 μg/lane of Recombinant Cynomolgus Monkey B7-H7/HHLA2 Fc Chimera (Catalog # 10108-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 92-109 kDa and 180-220 kDa, respectively.

Formulation, Preparation and Storage

10108-B7
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating protein 2), is a member of the B7 family of immune regulatory proteins (1, 2). The mature human B7-H7 protein consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). Within the ECD, cynomolgus B7-H7 shares 90% aa sequence identity with human B7-H7. It is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). The receptor TMIGD2, commonly found in endothelial and epithelial cells, has also been identified as one of the receptors for B7-H7 (6). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

References

  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
  6. Janakiram, M. et al. (2015) OncoImmunology 4:e1026534-1

Long Name

B7 Homolog 7

Alternate Names

B7H7, HHLA2

Entrez Gene IDs

11148 (Human); 102137704 (Cynomolgus Monkey)

Gene Symbol

HHLA2

UniProt

XP_005548285

Additional B7-H7/HHLA2 Products

Product Documents for Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Cynomolgus B7-H7/HHLA2 Fc Chimera Protein, CF

For research use only

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