Recombinant Cynomolgus CD200R1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9357-CD

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

XP_005548208

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD200R1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD200 R1 protein
Human Cynomolgus Monkey CD200 R1
(Ala27-Leu267)
Accession # XP_005548208		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

53 kDa

SDS-PAGE

85-102 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CD200 R1 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) that produces 50% of the optimal binding response is 1.5-9 ng/mL.

Scientific Data Images for Recombinant Cynomolgus CD200R1 Fc Chimera Protein, CF

Recombinant Cynomolgus CD200R1 Fc Chimera Protein Bioactivity

Recombinant Cynomolgus CD200R1 Fc Chimera Protein Bioactivity

When Recombinant Cynomolgus CD200 R1 Fc Chimera (Catalog # 9357-CD) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human CD200 Fc Chimera Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD) binds with an ED50 of 1.5-9 ng/mL.

Formulation, Preparation and Storage

9357-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 1 mg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa transmembrane protein in the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1-3). The cynomolgus CD200 R1 cDNA encodes a 241 aa extracellular domain (ECD) and 61 aa cytoplasmic tail. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, cynomolgus CD200 R1 shares 91%, 54%, and 57% aa sequence identity with human, mouse, and rat CD200 R1, respectively. At least two alternate splice isoforms exist that differ in their cytoplasmic domains. CD200 R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells (5-7), while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand system by knockout of the CD200 gene in mice leads to increased macrophage number and activation and predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). CD200 R1 propagates inhibitory signals despite its lacking a cytoplasmic ITIM (immunoreceptor tyrosine-based inhibitory motif) (6, 7, 11, 12).

References

  1. Rosenblum, M.D. et al. 2006, J. Dermatol. Sci. 41:165.
  2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
  3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
  4. Wright, G.J. et al. (2003) J. Immunol. 171:3034.
  5. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
  6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
  7. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
  8. Wright, G.J. et al. (2001) Immunology 102:173.
  9. Hoek, R.M. et al. (2000) Science 290:1768.
  10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  11. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  12. Zhang, S. et al. (2004) J. Immunol. 173:6786.

Long Name

CD200 Receptor 1

Alternate Names

CD200 R1, MOX2R

Entrez Gene IDs

131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)

Gene Symbol

CD200R1

UniProt

XP_005548208

Additional CD200R1 Products

Product Documents for Recombinant Cynomolgus CD200R1 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus CD200R1 Fc Chimera Protein, CF

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