Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9297-DC

R&D Systems, part of Bio-Techne
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9297-DC-100
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Key Product Details

Source

CHO

Accession #

EHH65162

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all DcR3/TNFRSF6B Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey DcR3/TNFRSF6B protein
Cynomolgus Monkey DcR3/TNFRSF6B
(Ala30-His300)
Accession # EHH65162		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala30

Predicted Molecular Mass

56 kDa

SDS-PAGE

58-67 kDa, reducing conditions

Activity

Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759.
The ED50 for this effect is 15‑75 ng/mL in the presence of 20 ng/mL Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL).

Scientific Data Images for Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein, CF

Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein Bioactivity

Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein Bioactivity

Recombinant Cynomolgus Monkey DcR3/TNFRSF6B Fc Chimera (Catalog # 9297-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 15-75 ng/mL.

Formulation, Preparation and Storage

9297-DC
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 500 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DcR3/TNFRSF6B

DcR3 (Decoy Receptor 3), also known as TR6 and TNFRSF6B is a 35-40 kDa secreted member of the TNF Receptor superfamily (1). Mature cynomolgus DcR3 contains four tandem TNFR cysteine-rich domains and shares 92% amino acid sequence identity with human DcR3. It binds to the TNF superfamily ligands Fas Ligand, TL1A, and LIGHT (2-5) and interferes with their respective interactions with Fas, DR3, or HVEM and Lymphotoxin betaR (2-4). It blocks apoptosis triggered through either Fas Ligand, TL1A, or LIGHT (2, 3, 5, 6). DcR3 is up-regulated in a variety of cancers and enhances tumor cell immune evasion (2, 3, 7). It also promotes immune suppression by inducing dendritic cell apoptosis (8), inhibiting NK cell and CD8+ T cell activity (2, 4), and inhibiting the production of inflammatory cytokines during viral infection or autoimmunity (9, 10). In humans, proteolytic removal of the C-terminal 53 amino acids generates a shortened DcR3 that retains the ability to block LIGHT but not Fas Ligand induced apoptosis (11). DcR3 can also induce osteoclast formation from monocytes (12).

References

  1. Siakavellas, S.I. and G. Bamias (2015) Inflamm. Bowel Dis. 21:2441.
  2. Pitti, R.M. et al. (1998) Nature 396:699.
  3. Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733.
  4. Zhang, J. et al. (2001) J. Clin. Invest. 107:1459.
  5. Migone, T.S. et al. (2002) Immunity 16:479.
  6. Han, B. and J. Wu (2009) J. Immunol. 183:8157.
  7. Ge, Z. et al. (2011) Exp. Ther. Med. 2:167.
  8. You, R.I. et al. (2008) Blood 111:1480.
  9. Huang, M.T. et al. (2015) J. Mol. Med. (Berlin) 93:1131.
  10. Mueller, A.M. et al. (2009) J. Neuroimmunol. 209:57.
  11. Wroblewski, V.J. et al. (2003) Biochem. Pharmacol. 65:657.
  12. Yang, C.R. et al. (2004) Cell Death Differ. 11 Suppl 1:S97.

Long Name

Decoy Receptor 3

Alternate Names

M68, TNFRSF6B, TR6

Entrez Gene IDs

8771 (Human); 107126607 (Cynomolgus Monkey)

Gene Symbol

TNFRSF6B

UniProt

EHH65162

Additional DcR3/TNFRSF6B Products

Product Documents for Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus DcR3/TNFRSF6B Fc Chimera Protein, CF

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