Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9240-CD

R&D Systems, part of Bio-Techne
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9240-CD-100
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Key Product Details

Source

HEK293

Accession #

NP_001270598

Conjugate

Unconjugated

Applications

Bioactivity

View all Fc gamma RIIA/CD32a Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RIIA/CD32a protein
Thr29-Ile211, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr29

Predicted Molecular Mass

21 kDa

SDS-PAGE

23-32 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey Fc gamma RIIA/CD32a is immobilized on a His Tag Antibody coated plate, it binds Biotinylated Human IgG. The concentration of Biotinylated Human IgG that produces 50% of the optimal binding response is approximately
0.75-3.75 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF

Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein Bioactivity

Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein Bioactivity

When Recombinant Cynomolgus Monkey Fc gamma RIIA/CD32a (Catalog # 9240-CD) is immobilized on a His Tag Antibody coated plate, Biotinylated Human IgG binds with an ED50 of 0.75-3.75 μg/mL.

Formulation, Preparation and Storage

9240-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIA/CD32a

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1, 2). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. CD32 proteins are encoded by three genes in human (Fc gamma RII A, B, and C), two in cynomolgus (Fc gamma RII A and B), and one in mouse (Fc gamma RIIB). Fc gamma RII A and C contain ITAM sequences, while Fc gamma RIIB contains an ITIM sequence in their cytoplasmic domains. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1, 3). Mature cynomolgus Fc gamma RIIA consists of a 183 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 77 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RIIA shares 89% aa sequence identity with human Fc gamma RIIA. It binds cynomolgus and human IgG subclasses 1-4 as well as human Pentraxin-2, and C-Reactive Protein (4, 5). It delivers activating signals through its cytoplasmic ITAM sequence (6). Fc gamma RIIA signaling cooperates with Integrin alpha2b beta3 and the CD42b-GP1b-IX-V complex to promote platelet adhesion and activation (7, 8). Fc gamma RIIA is expressed on monocytes, monocytes, and platelets and additionally on cynomolgus granulocytes (1, 4).

References

  1. Qiao, J. et al. (2015) Immunol. Rev. 268:241.
  2. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Warncke, M. et al. (2012) J. Immunol. 188:4405.
  5. Lu, J. et al. (2008) Nature 456:989.
  6. Rosenfeld, S.I. et al. (1985) J. Clin. Invest. 76:2317.
  7. Sullam, P.M. et al. (1998) J. Biol. Chem. 273:5331.
  8. Boylan, B. et al. (2008) Blood 112:2780.

Long Name

Fc gamma Receptor II A

Alternate Names

CD32a, FCGR2A, FcgRIIA, FCRIIA

Entrez Gene IDs

2212 (Human); 14130 (Mouse); 116591 (Rat); 102143568 (Cynomolgus Monkey)

Gene Symbol

FCGR2A

UniProt

NP_001270598

Additional Fc gamma RIIA/CD32a Products

Product Documents for Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF

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