Recombinant Cynomolgus Mesothelin (aa417-693) Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10760-MS

C-terminal
R&D Systems, part of Bio-Techne
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10760-MS-050
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Key Product Details

Source

CHO

Accession #

XP_015298164.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Mesothelin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Mesothelin protein
Cynomolgus Monkey Mesothelin
(Asp417-Asp693)
Accession # XP_015298164.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp417

Predicted Molecular Mass

58 kDa

SDS-PAGE

65-85 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CA125/MUC16 (Catalog # 5609-MU) is immobilized at 0.5 ug/mL (100 µL/well), Recombinant Cynomolgus Monkey Mesothelin C-Terminal (aa 417-693) Fc Chimera binds with an ED50 of 4.0-20.0 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Mesothelin (aa417-693) Fc Protein, CF

Recombinant Cynomolgus Monkey Mesothelin (aa 417-693) Fc Chimera Protein Binding Activity.

When Recombinant Human CA125/MUC16 (5609-MU) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Cynomolgus Mesothelin (aa 417-693) Fc Chimera (Catalog # 10760-MS) binds with an ED50 of 4.0-20.0 ng/mL.

Recombinant Cynomolgus Monkey Mesothelin (aa 417-693) Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey Mesothelin (aa 417-693) Fc Chimera (Catalog # 10760-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-85 kDa and 130-170 kDa, respectively.

Formulation, Preparation and Storage

10760-MS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and overexpressed in several human tumors (1, 2). The mesothelin gene encodes a ~70 kDa precursor protein that is cleaved at a dibasic proteolytic site into a 40-kDa membrane-bound protein termed MSLN and a 31-kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell (3, 4). Cleaved cynomolgus MSLN remains attached to the cell surface via a GPI linkage and shares 84% amino acid sequence identity with human MSLN. In human, alternate splicing generates additional MSLN isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor (3). Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 4). It is upregulated in mesotheliomas and a range of carcinomas and adenomas (5-7). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression (8, 9). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (10). A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions (10-12).

References

  1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
  2. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
  3. Muminova, Z.E. et al. (2004) BMC Cancer 4:19.
  4. Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
  5. Ordonez, N.G. (2003) Mod. Pathol. 16:192.
  6. Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
  7. Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
  8. Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
  9. Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
  10. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
  11. Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
  12. Robinson, B.W.S. et al. (2003) Lancet 362:1612.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

XP_015298164.1

Additional Mesothelin Products

Product Documents for Recombinant Cynomolgus Mesothelin (aa417-693) Fc Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus Mesothelin (aa417-693) Fc Protein, CF

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