Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10197-CD

R&D Systems, part of Bio-Techne
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10197-CD-050
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Key Product Details

Source

CHO

Accession #

EHH50231

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD160 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD160 protein
Cynomolgus Monkey CD160
(Ile27-Ser159)
Accession # EHH50231		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile27

Predicted Molecular Mass

41 kDa

SDS-PAGE

40-60 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey CD160 Fc Chimera that produces 50% of the optimal binding response 1.5-9 ng/mL

Scientific Data Images for Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein Binding Activity

Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein Binding Activity

When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 µg/mL, Recombinant Cynomolgus Monkey CD160 Fc Chimera (Catalog # 10197-CD) binds with an ED50 of 1.5-9 ng/mL.
Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey CD160 Fc Chimera (Catalog # 10197-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-60 kDa and 80-120 kDa, respectively.

Formulation, Preparation and Storage

10197-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD160

CD160, also known as Natural killer cell receptor BY55, is a GPI-anchored member of the Ig superfamily that is expressed on both cytolytic lymphocytes and some unstimulated CD4+ T cells (1-4). In human, it is expressed principally on non-myeloid hematopoietic cells (1, 5-7). It is synthesized as a preproprotein with 181 amino acids including a 24 amino acid (aa) signal sequence, a 135 aa CD160 chain that contain one 96 aa V‑type Ig‑like domain, and a 22 aa propeptide that is cleaved to generate a GPI‑linkage at Ser159. The GPI‑linked CD160 is known to be cleaved by phospholipases and generate an 80 kDa (presumably trimeric) band in SDS‑PAGE (1, 8).  Mature cynomolgus CD160 shares 91% aa sequence identity with human CD160. CD160 is known to bind to HLA-G1, HLA-C, and HVEM (6, 9, 10). Upon engagement, it is reported to associate with CD2 in cis under certain conditions (11, 12). The effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on CD56DIM NK cells, CD160 signaling in response to HLA-C binding promotes IFN-gamma, TNF-alpha, and IL-6 secretion (10). When up-regulated on CD4+ T cells following activation, CD160 engagement by HVEM (expressed by APC) serves to block a simultaneous LIGHT stimulation of HVEM that promotes receptor expression and cytokine release (1, 2, 7, 13).

References

  1. Cai, G. & G.J. Freeman (2009) Immunol. Rev. 229:244.
  2. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
  3. Maiza, H. et al. (1993) J. Exp. Med. 178:1121.
  4. Anumanthan, A. et al. (1998) J. Immunol. 161:2780.
  5. Abecassis, S. et al. (2007) J. Invest. Dermatol. 127:1161.
  6. Fons, P. et al. (2006) Blood 108:2608.
  7. Kaye, J. et al. (2008) Nat. Immunol. 9:122.
  8. Giustiniani, J. et al. (2007) J. Immunol. 178:1293.
  9. Giustinani, J. et al. (2009) J. Immunol. 182:63.
  10. Barakonyi, A. et al. (2004) J. Immunol. 173:5349.
  11. Nikolova, M. et al. (2002) Int. Immunol. 14:445.
  12. Rabot, M. et al. (2006) Transpl. Immunol. 17:36.
  13. Cai, G. et al. (2008) Nat. Immunol. 9:176.

Alternate Names

BY55, CD160, NK1, NK28

Entrez Gene IDs

11126 (Human); 54215 (Mouse); 502585 (Rat); 102117412 (Cynomolgus Monkey)

Gene Symbol

CD160

UniProt

EHH50231

Additional CD160 Products

Product Documents for Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein, CF

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