Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11122-MK

R&D Systems, part of Bio-Techne
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11122-MK-050
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Key Product Details

Source

CHO

Structure / Form

Disulfide-linked homodimer.
Biotinylated via Avi-tag.

Conjugate

Unconjugated

Applications

Bioactivity

View all MuSK Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey MuSK protein
Cyno MuSK
(Leu24-Thr495)
Accession # XP_005581150.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24

Predicted Molecular Mass

78 kDa

SDS-PAGE

85-105 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
In the presence of Recombinant Human Agrin  (Catalog # 6624-AG), immobilized Recombinant Cynomolgus Monkey MuSK Fc Chimera  (Catalog # 11122-MK) at 0.5 µg/mL (100 µL/well) binds Recombinant Human LRP-4  (Catalog # 5948-LR) with an ED50 of 10.0-80.0 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein Binding Activity.

In the presence of Recombinant Human Agrin (6624-AG), immobilized Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein (Catalog # 11122-MK) at 0.5 µg/mL (100 µL/well) binds Recombinant Human LRP-4 (5948-LR) with an ED50 of 10.0-80.0 ng/mL.

Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein (Catalog # 11122-MK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa and 170-210 kDa, respectively.

Formulation, Preparation and Storage

11122-MK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MuSK

Muscle-specific kinase (MuSK), also known as muscle skeletal receptor tyrosine-protein kinase, is a single-pass transmembrane protein belonging to the protein kinase superfamily (1). Cynomolgus MuSK consists of an extracellular domain (ECD) with 3 immunoglobulin-like domains and a cysteine-rich domain (CRD), a transmembrane domain, and a cytoplasmic domain with a tyrosine kinase domain (2). Within the mature ECD, cynomolgus MuSK shares 99% and 91% amino acid sequence identity with human and mouse MuSK, respectively. Alternative splicing of MuSK results in multiple isoforms which could result in altered properties and functions (3, 4). MuSK is expressed by skeletal muscle cells and excitatory neurons in the central nervous system (CNS) (1, 2). MuSK is essential for neuromuscular synapse formation and activation of the MuSK signaling cascade is critical for proper signaling between motor neurons and skeletal muscle (1-4). Once activated, MuSK stimulates the pathways that facilitate transcription of genes which encode synaptic proteins in muscle, activate retrograde signaling which promotes presynaptic differentiation, and cluster and anchor acetylcholine receptors (AChRs) (1, 5). Low-density lipoprotein receptor-related protein-4 (Lrp4), is the ligand for MuSK, and its binding affinity is potentiated by agrin (3). Mutant mice lacking agrin, MuSK, and Lrp4 fail to form neuromuscular junctions (NMJ) and subsequently died at birth due to respiratory failure (2, 3). In the presence of mutations which impair MuSK kinase activity or downstream signaling from MuSK, synapses become both structurally and functionally defective, leading to congenital myasthenia (6). The autoimmune disease myasthenia gravis is another neuromuscular disease caused by autoantibodies to AChRs, MuSK or Lrp4 (6). Our Avi-tag Biotinylated MuSK features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity

References

  1. Burden, S.J. et al. (2013) CSH Perspectives Bio. 5(5).
  2. Hubbard, S.R. and Gnanasambandan, K. (2013) Biochem Biophys Acta. 1834(10):2166.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. Kuehn, R. et al. (2005) Gene 360:83.
  5. Camurdanoglu, B.Z. et al. (2016) Sci. Rep. 6:33583.
  6. Herbst, R. (2020) Neurosci. Lett. (2020) 716:134676.

Long Name

Muscle-specific Receptor Tyrosine Kinase

Alternate Names

EC 2.7.10, EC 2.7.10.1, MGC126323, MGC126324, muscle, skeletal, receptor tyrosine kinase, MuSK, skeletal receptor tyrosine-protein kinase

Entrez Gene IDs

4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)

Gene Symbol

MUSK

Additional MuSK Products

Product Documents for Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Cynomolgus Monkey MuSK Fc Chimera Protein, CF

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