Recombinant Cynomolgus Monkey TIM-4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9384-TM

R&D Systems, part of Bio-Techne
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9384-TM-050
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Key Product Details

Source

HEK293

Accession #

XP_005558436

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TIM-4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey TIM-4 protein
Cynomolgus Monkey TIM-4
(Glu25-Gln305)
Accession # XP_005558436		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu25

Predicted Molecular Mass

57 kDa

SDS-PAGE

95-112 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 0.2-1.2 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey TIM-4 Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey TIM-4 Fc Chimera Protein Bioactivity

Recombinant Cynomolgus Monkey TIM-4 Fc Chimera Protein Bioactivity

Recombinant Cynomolgus Monkey TIM-4 Fc Chimera (Catalog # 9384-TM) inhibits anti-CD3-induced proliferation of human T cells. The ED50 for this effect is 0.2-1.2 μg/mL.

Formulation, Preparation and Storage

9384-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is an approximately 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1-3). Sequence alignment with the human TIM-4 shows that mature cynomolgus monkey TIM-4 has a predicted 280 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 43 aa cytoplasmic tail. Within the ECD, cynomolgus monkey TIM-4 shares 87 % and 48 % aa sequence identity with human and mouse TIM-4, respectively. Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1or TIM-3. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (2, 3). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (3, 4). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (3, 5). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (3).

References

  1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  2. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
  3. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  4. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  5. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Long Name

T Cell Immunoglobulin Mucin-4

Alternate Names

Smuckler, TIM4, TIMD4

Entrez Gene IDs

91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)

Gene Symbol

TIMD4

UniProt

XP_005558436

Additional TIM-4 Products

Product Documents for Recombinant Cynomolgus Monkey TIM-4 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus Monkey TIM-4 Fc Chimera Protein, CF

For research use only

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