Recombinant Cynomolgus PD-L2/B7-DC Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9178-PL

R&D Systems, part of Bio-Techne
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9178-PL-01M
9178-PL-100
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Key Product Details

Source

HEK293

Accession #

XP_005581839

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all PD-L2/B7-DC Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey PD-L2/B7-DC protein
Cynomolgus Monkey PD-L2/B7-DC
(Leu20-Pro219)
Accession # XP_005581839		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

49 kDa

SDS-PAGE

62-77 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey PD‑L2/B7‑DC Fc Chimera that produces 50% of the optimal binding response is approximately 0.1-0.6 μg/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 9178-PL in the following applications:

Scientific Data Images for Recombinant Cynomolgus PD-L2/B7-DC Fc Chimera Protein, CF

Recombinant Cynomolgus PD-L2/B7-DC Fc Chimera Protein Bioactivity

Recombinant Cynomolgus PD-L2/B7-DC Fc Chimera Protein Bioactivity

When Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) is coated at 1 µg/mL (100 μL/well), Recombinant Cynomolgus Monkey PD‑L2/B7‑DC Fc Chimera (Catalog # 9178-PL) binds with a typical ED50 of 0.1-0.6 μg/mL.

Formulation, Preparation and Storage

9178-PL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a transmembrane member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Within the ECD, cynomolgous PD-L2 shares 71% and 96% aa sequence identity with mouse and human PD-L2, respectively (2, 3). PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3-5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
  4. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  5. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  6. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  7. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  8. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  9. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  10. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  11. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  12. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  13. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.

Long Name

Programmed Death-1 Ligand-2

Alternate Names

B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2

Entrez Gene IDs

80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)

Gene Symbol

PDCD1LG2

UniProt

XP_005581839

Additional PD-L2/B7-DC Products

Product Documents for Recombinant Cynomolgus PD-L2/B7-DC Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus PD-L2/B7-DC Fc Chimera Protein, CF

For research use only

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