Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11176-HE

Fc Chimera
R&D Systems, part of Bio-Techne
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11176-HE-050
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Key Product Details

Source

CHO

Accession #

XP_005562748.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Hepassocin/FGL1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived Hepassocin/FGL1 protein
MD Human IgG1 Fc
(Pro100-Lys330)		 IEGR Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1
(Ala63-Ile348)
Accession # XP_005562748.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met (of Fc)

Predicted Molecular Mass

60 kDa

SDS-PAGE

58-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LAG-3 Fc Chimera Protein (Catalog # 2319-L3) is immobilized at 2.50 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera  (Catalog # 11176-HE) binds with an ED50 of 0.500-4.00 µg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CF

Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera Protein (2319-L3) is immobilized at 2.50 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein (Catalog # 11176-HE) binds with an ED50 of 0.500-4.00 µg/mL.

Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein (Catalog # 11176-HE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-65 kDa and 120-130 kDa, respectively.

Formulation, Preparation and Storage

11176-HE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Glycerol with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as Hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1) (1), is a liver-specific secreted protein belonging to the fibrinogen superfamily whose members share a fibrinogen domain at their C-termini (2). Human Hepassocin/FGL1 is a secreted homodimer consisting of 312 amino acids (aa) with a 22 aa signal sequence and a 290 aa mature protein (3). Cynomolgus monkey shares 100% and 96.5% amino acid identity with rhesus macaque and human Hepassocin/FGL1, respectively. Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II (4). Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses (4). Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells. It is upregulated during liver regeneration following partial hepatectomy (5), and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats (6). Its expression is regulated in Hep G2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant (7).

References

  1. Yamamoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 2:681.
  2. Zhang, S.M. et al. (2008) Innate Immun. 14:175.
  3. Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
  4. Wang, J. et al. (2019) Cell. 176:334.
  5. Yu, HT. et al. (2009) J. Biol. Chem. 284:13335.
  6. Li, C.Y. et al. (2010) Gut. 59:817.
  7. Liu, Z. and C. Ukomadu. (2008) Biochem. Briophys. Res. Commun. 365:729.

Long Name

Fibrinogen Like 1

Alternate Names

FGL1, Fibrinogen-like-protein 1, FREP1

Entrez Gene IDs

2267 (Human); 234199 (Mouse); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Gene Symbol

FGL1

UniProt

XP_005562748.1

Additional Hepassocin/FGL1 Products

Product Documents for Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus/Rhesus Hepassocin/FGL1 Fc Protein, CF

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