Recombinant Cynomolgus/Rhesus Macaque TrkA His Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11317-TK

R&D Systems, part of Bio-Techne
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11317-TK-050
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Key Product Details

Source

CHO

Accession #

XP_005541454.3

Conjugate

Unconjugated

Applications

Bioactivity

View all TrkA Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived TrkA protein
Ser34-Gly423, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser34

Predicted Molecular Mass

43 kDa

SDS-PAGE

75-90 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.0800-0.800 μg/mL in the presence of 10 ng/mL of Recombinant Human beta-NGF Protein  (Catalog # 256-GF).

Scientific Data Images for Recombinant Cynomolgus/Rhesus Macaque TrkA His Protein, CF

Recombinant Cynomolgus Monkey/Rhesus Macaque TrkA His-tag Protein Bioactivity.

Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.0800-0.800 μg/mL in the presence of 10 ng/mL of Recombinant Human beta-NGF Protein (256-GF).

Recombinant Cynomolgus/Rhesus Macaque TrkA His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus/Rhesus Macaque TrkA His-tag Protein (Catalog # 11317-TK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-90 kDa.

Formulation, Preparation and Storage

11317-TK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA (Tyrosine kinase receptor A), also known as High affinity NGF receptor, is a member of the neurotrophic tyrosine kinase receptor family that has three members,  TrkA, Trk B and Trk C, which preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively (1). All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues (1). Mature cynomolgus TrkA consists of a 391 amino acid (aa) extracellular domain (ECD) which shares 98.5% aa identity with human TrkA.  NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily (2). NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when being expressed independent of Trk (3).

References

  1. Shelton D.L. et al. (1995) J. Neurosci. 15:477.
  2. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  3. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat); 102129364 (Cynomolgus Monkey)

Gene Symbol

NTRK1

UniProt

XP_005541454.3

Additional TrkA Products

Product Documents for Recombinant Cynomolgus/Rhesus Macaque TrkA His Protein, CF

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Product Specific Notices for Recombinant Cynomolgus/Rhesus Macaque TrkA His Protein, CF

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