Recombinant Drosophila Notch EGF20 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8117-DN

R&D Systems, part of Bio-Techne
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8117-DN-200
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Key Product Details

Source

E. coli

Accession #

P07207

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Notch EGF20 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived drosophila Notch EGF20 protein
Leader Sequence
SPWPLD		 Notch
(Asp791-Asn829)
Accession # P07207		 Linker 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser

Predicted Molecular Mass

8.4 kDa

SDS-PAGE

9 kDa, reducing conditions

Activity

Measured by its ability to be the acceptor substrate for Recombinant Mouse EOGT (Catalog # 8116-GT).
The specific activity of Recombinant Mouse EOGT is >0.7 pmol/min/ug using Notch Peptide as an acceptor and measured under the described conditions.

Formulation, Preparation and Storage

8117-DN
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Notch EGF20

Drosophila notch protein is a signaling protein that regulates the differentiation of eye, wing disk, oogenesis, segmental appendages and nervous system through lateral inhibition or induction. It functions as a receptor for membrane-bound ligands Delta and Serrate to regulate cell-fate determination (1). The mature protein is a transmembrane protein with an extracellular domain from amino acid 53 to 1745, which consists of 36 EGF repeats (1). The EGF repeats are modified with O-linked beta-N-acetylglucosamine (O-GlcNAc) by an extracellular O-GlcNAc transferase (EOGT) (2, 3). The EGF repeat 20 expressed in this construct can be used as a substrate for EOGT (recombinant mouse EOGT, R&D catalogue 8116-GT) (2). It may also be used as a substrate for protein O-Glucosyltransferase 1 (POGLUT) (4) (recombinant human POGLUT, R&D catalogue 6437-GT) and Fringe (5).

References

  1. Wharton K.A. et al. (1985) Cell 43:567-581.
  2. Matsuura A. et al.   (2008) J. Biol. Chem. 283:35486.
  3. Sakaidani, Y. et al. (2012)  Biochem. Biophys. Res. Commun. 419:14.
  4. Acar, M. et al. (2008) Cell 132:247.
  5. Bruckner K. et al. (2000) Nature 406:411.

Long Name

Notch EGF repeat 20

Entrez Gene IDs

31293 (Drosophila)

Gene Symbol

N

UniProt

P07207

Additional Notch EGF20 Products

Product Documents for Recombinant Drosophila Notch EGF20 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Drosophila Notch EGF20 Protein, CF

For research use only

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