Recombinant F. meningosepticum PNGase F Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9109-GH
Effective removal of N-linked oligosaccharides from glycoproteins. Glycerol-free, suitable for downstream MS and HPLC.
Key Product Details
Product Specifications
Source
E. coli-derived f. meningosepticum PNGase F protein
Ala41-Asn354 with N-terminal Met and 6-His tag
Ala41-Asn354 with N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
36 kDa
SDS-PAGE
34 kDa, under reducing conditions.
Activity
Measured by its ability to deglycosylate ribonuclease B under denatured conditions.
>50% ribonuclease B (10 μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.
>50% ribonuclease B (10 μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.
Scientific Data Images for Recombinant F. meningosepticum PNGase F Protein, CF
Recombinant F. meningosepticum PNGase F Protein Enzyme Activity Diagram.
Recombinant F. meningosepticum PNGase F Protein, CF (Catalog # 9109-GH) is an amidase that cleaves the amide bond between the GlcNAc residue of an N-glycan and the underlying asparagine residue. Only the pentasaccharide core of an N-glycan is pictured here for clarity. The pentasaccharide can be extended at R1, R2 and R3 positions. R1 can be an alpha-6 fucose but not an alpha-3 fucose. R2 and R3 can be any of common monosaccharides or oligosaccharides.
Recombinant F. meningosepticum PNGase F Protein Bioactivity
Total activity per vial of Recombinant F. meningosepticum PNGase F (Catalog # 9109-GH) compared to the leading competitor. R&D Systems®PNGase F gives you 2x more enzyme at a comparable price.
Recombinant F. meningosepticum PNGase F Protein Enzyme Activity.
RecombinantF. meningosepticumPNGase F (Catalog # 9109-GH) from R&D Systems and a leading competitor are able to deglycosylate 10 μg of RNase B at 37 °C in one hour. TheE. coli-produced enzyme from R&D Systems offers a better value than the competition.Formulation, Preparation and Storage
9109-GH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PNGase F
beta-aspartylglucosamine bond between the innermost GlcNAc of N-glycans and asparagine residues of glycoproteins (2). The enzyme is highly active on various
N-glycans except those with the innermost GlcNAc modified with alpha 1-3-linked core fucose, which is commonly found on plant glycoproteins (3). Cleavage with PNGase F will convert the asparagine residue to an aspartic residue, allowing identification of the glycosylation site by mass spectrometry (4). This purified enzyme is compatible with glycan analysis using mass spectrometry.
References
- Elder, J.H. and Alexander, S. (1982) Proc. Natl. Acad. Sci. USA 79:4540.
- Maley, F. et al. (1989) Anal. Biochem. 180:195.
- Tarentino, A.L. and Plummer, T.H. (1994) Methods Enzymol 230:44.
- Zhang, H. et al. (2003) Nat. Biotechnol. 21:660.
Long Name
Peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine Amidase F
Alternate Names
PNGF
UniProt
Additional PNGase F Products
Product Documents for Recombinant F. meningosepticum PNGase F Protein, CF
Product Specific Notices for Recombinant F. meningosepticum PNGase F Protein, CF
For research use only
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