Recombinant His6-SARS Virus Papain-like Protease Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E-610

R&D Systems, part of Bio-Techne
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E-610-050
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Key Product Details

Source

E. coli

Accession #

P0C6U8.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Papain-like Protease Proteins and Enzymes »

Product Specifications

Source

E. coli-derived viral Papain-like Protease protein
Glu1541 - Lys1855 with a N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

37 kDa

Activity

Reaction conditions will need to be optimized for each specific application.  We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-AMC (U-550) or Ubiquitin-Rhodamine 110 (U-555) as a subtrate.  

Formulation, Preparation and Storage

E-610
Formulation Supplied as a solution in HEPES, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Papain-like Protease

The Papain-like protease ("PLPro") from the human SARS coronavirus (Severe Acute Respiratory Syndrome coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide.  PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region.  In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity.  In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.  PLPro has been reported to hydrolyze both K48- and K63 linked poly-Ubiquitin chains in vitro.  When used at low concentrations, the enzyme demonstrates a strong preference for K48-linked tetra-Ubiquitin chains which are primarily converted to di-Ubiquitin species.  

References

  1. Frieman M., et al.  (2009) J.Virol.  83: 6689.
  2. Lindner H.A.,et al.  (2007) Archv. Biochem. Biophys.  466: 8.
  3. Ratia K., et al. (2014) PLoS Pathog. 10:e1004113.
  4. Russell N., et al. (2016) FASEB Ubiquitin & Cell Reg.  (poster)
 

Alternate Names

PL-PRO, PLpro, pp1a, Replicase polyprotein 1a

UniProt

P0C6U8.1

Additional Papain-like Protease Products

Product Documents for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant His6-SARS Virus Papain-like Protease Protein, CF

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