Recombinant Human 15-PGDH/HPGD Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5660-DH
Key Product Details
Source
E. coli
Accession #
Structure / Form
Noncovalently-linked homodimer
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
E. coli-derived human 15-PGDH/HPGD protein
Met1-Gln266, with a C-terminal 6-His tag
Met1-Gln266, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1
Predicted Molecular Mass
30 kDa
SDS-PAGE
28 kDa, reducing conditions
Activity
Measure by the production of NADH during the oxidation of PGF2 alpha.
The specific activity is >1,500 pmol/min/μg, as measured under the described conditions.
The specific activity is >1,500 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
5660-DH
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: 15-PGDH/HPGD
HPGD, or 15-hydroxyprostaglandin dehydrogenase, is a NAD+-linked dehydrogenase that oxidizes the hydroxyl group at position 15 of prostaglandins to a ketone, resulting in a loss of biological activity (1). HPGD is a major enzyme for the catabolism of prostaglandins. The enzyme is a member of the short-chain alcohol dehydrogenase family of enzymes (2). HPGD is a cytosolic enzyme expressed in most tissues, with highest expression levels in placenta, lung, and kidney (3). It is inhibited by aspirin and nonsteroidal anti-inflammatory drugs (4). Defects in HPGD are a cause of hypertrophic osteoarthropathy (5).
References
- Anggard, E. and B. Samuelsson (1964) J. Biol. Chem. 239:4097.
- Krook, M. et al. (1990) Biochemistry 29:738.
- Tai, H.H. (1976) Biochemistry 15:4586.
- Mak, O.T. et al. (1982) Biosci. Rep. 2:503.
- Uppal, S. et al. (2008) Nat. Genet. 40:789.
Long Name
15-Hydroxyprostaglandin Dehydrogenase
Alternate Names
15PGDH, HPGD, PGDH1, SDR36C1
Gene Symbol
HPGD
UniProt
Additional 15-PGDH/HPGD Products
Product Documents for Recombinant Human 15-PGDH/HPGD Protein, CF
Product Specific Notices for Recombinant Human 15-PGDH/HPGD Protein, CF
For research use only
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