Recombinant Human 5T4 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI4975

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Q13641.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all 5T4 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human 5T4 protein
Human 5T4
(Ser31-Ser355)
Accession # Q13641.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser31

Predicted Molecular Mass

64 kDa

SDS-PAGE

90-105 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human 5T4 Antibody (Catalog # MAB49751) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human 5T4 Fc Chimera Avi-tag binds with an ED50 of 0.45-3.60 ng/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human Frizzled-8 Fc Chimera  (Catalog # 6129-FZ) is immobilized at 5 µg/mL (100 µL/well), Biotinylated Recombinant Human 5T4 Fc Chimera Avi-tag (Catalog # AVI4975) binds with an ED50 of 5.00-40.0 µg/mL.

Scientific Data Images for Recombinant Human 5T4 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human 5T4 Fc Chimera Avi-tag Protein Binding Activity.

When Human 5T4 Antibody (MAB49751) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human 5T4 Fc Chimera Avi-tag (Catalog # AVI4975) binds with an ED50 of 0.45-3.60 ng/mL.

Biotinylated Recombinant Human 5T4 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human 5T4 Fc Chimera Avi-tag (Catalog # AVI4975) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-105 kDa and 180-210 kDa, respectively.

Formulation, Preparation and Storage

AVI4975
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 5T4

5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling (1). Mature 5T4 consists of an extracellular domain (ECD) with eight leucine-rich repeats thought to antagonize Wnt signaling, a single transmembrane segment, and a short cytoplasmic tail with a PDZ-binding motif (2). Within the ECD, human 5T4 shares 78% and 77% amino acid sequence identity with mouse and rat 5T4, respectively. 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis (3-5). It has been identified that 5T4 interacts with TIP-2/GIPC, a common interacting protein involved in cancer, through its PDZ domain (6). 5T4 inhibits Wnt/ beta -catenin signaling by binding to LRP6 and inhibits Wnt-induced LRP6 internalization into endocytic vesicles (1). Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1 (1). In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled-8. Our Avi-tag Biotinylated 5T4 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Kagermeier-Schenk, B. et al. (2011) Dev. Cell. 21:1129.
  2. Zhao, Y. et al. (2014) Structure. 22:612.
  3. Starzynska, T. et al. (1994) Br. J. Cancer. 69:899.
  4. Naganuma, H. et al. (2002) Anticancer Res. 22:1033.
  5. Harris, J.L. et al. (2018) Int. J. Biochem. Cell Biol. 99:28.
  6. Awan, A. et al. (2002) BBRC 290:1030.

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)

Gene Symbol

TPBG

UniProt

Q13641.1

Additional 5T4 Products

Product Documents for Recombinant Human 5T4 Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human 5T4 Fc Chimera Avi-tag Protein, CF

For research use only

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