Recombinant Human ABP1/AOC1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 8298-AO
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ABP1/AOC1 protein
Glu20-Val751, with a N-terminal 6-His tag
Glu20-Val751, with a N-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Inconclusive, His predicted. Protein identity confirmed by MS analysis of tryptic fragments.
Predicted Molecular Mass
84 kDa
SDS-PAGE
83-92 kDa, reducing conditions
Activity
Measured by its ability to produce hydrogen peroxide during the oxidation of histamine.
The specific activity is >40 pmol/min/μg, as measured under the described conditions.
The specific activity is >40 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
8298-AO
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ABP1/AOC1
Amiloride binding protein (ABP1), also known as diamine oxidase (DAO) and amine oxidase copper domain-containing protein 1 (AOC1), is an approximately 110 kDa member of the copper-containing amine oxidase family (1, 2). ABP1 is a glycosylated enzyme that forms disulfide-linked homodimers (3). It is stored in cytoplasmic granules of epithelial cells in the kidney, placenta, uterus, lung, and intestine (1, 4), and its extracellular release can be induced by heparin (5). ABP1 exhibits a substrate preference for histamine and putrescine, generating hydrogen peroxide and ammonia in an oxidative deamination reaction (3). ABP1 activity plays an important role in the catabolism of histamine and other bioactive polyamines (6, 7). Deficiencies in ABP1 can result in histaminosis or dietary histamine intolerance (8, 9).
References
- Novotny, W.F. et al. (1994) J. Biol. Chem. 269:9921.
- Barbry, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7347.
- McGrath, A.P. et al. (2009) Biochemistry 48:9810.
- Liang, X.H. et al. (2010) Endocrinology 151:5007.
- Klocker, J. et al. (2004) Vascul. Pharmacol. 40:293.
- Jones, B.L. and G.L. Kearns (2011) Clin. Pharmacol. Ther. 89:189.
- Bieganski, T. et al. (1983) Biochim. Biophys. Acta 756:196.
- Sattler, J. and W. Lorenz (1990) J. Neural Transm. Suppl. 32:291.
- Maintz, L. and N. Novak (2007) Am. J. Clin. Nutr. 85:1185.
Long Name
Amiloride Binding Protein 1
Alternate Names
AOC1, Diamine Oxidase, Histaminase, KAO
Entrez Gene IDs
26 (Human)
Gene Symbol
AOC1
UniProt
Additional ABP1/AOC1 Products
Product Documents for Recombinant Human ABP1/AOC1 Protein, CF
Product Specific Notices for Recombinant Human ABP1/AOC1 Protein, CF
For research use only
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