Recombinant Human Active Akt1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1775-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Akt1 protein
Purity
>70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N terminal GST tag
SDS-PAGE
85 kDa
Activity
The specific activity of Akt1 was determined to be 189 nmol/min/mg as per activity assay protocol.
Reviewed Applications
Read 2 reviews rated 4 using 1775-KS in the following applications:
Scientific Data Images for Recombinant Human Active Akt1 Protein, CF
Recombinant Human Active Akt1 Protein SDS-PAGE
The approximate molecular weight is 85 kDa and the purity is > 70%.Formulation, Preparation and Storage
1775-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: Akt1
Akt1, also known as PKB alpha, is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). Akt1 is frequently over-expressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas, and prostate as well as lymphomas and leukemias (2).
References
- Coffer, P.J. et al. (1998) Biochem J. 335:1.2.
- Anderson, K.E. et al. (1998) Curr. Biol. 8:684.
Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 1
Alternate Names
PKB alpha, PRKBA, RAC-alpha
Gene Symbol
AKT1
UniProt
Additional Akt1 Products
Product Documents for Recombinant Human Active Akt1 Protein, CF
Product Specific Notices for Recombinant Human Active Akt1 Protein, CF
For research use only
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