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Recombinant Human Active Blk Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2679-KS

R&D Systems, part of Bio-Techne
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2679-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Blk protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N terminal GST tag

SDS-PAGE

84 kDa

Activity

The specific activity of Blk was determined to be 110 nmol/min/mg using a poly (Glu:Tyr, 4:1) synthetic peptide substrate (see Activity Assay Protocol).

Scientific Data Images for Recombinant Human Active Blk Protein, CF

Recombinant Human Active Blk Protein SDS-PAGE

Recombinant Human Active Blk Protein SDS-PAGE.

The approximate molecular weight is 84 kDa and the purity is > 90%.

Formulation, Preparation and Storage

2679-KS
Formulation Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: Blk

Blk, also known as B lymphoid kinase, is a 55 kDa tyrosine kinase with SH3, SH2, and catalytic domains that contain consensus sequences of the Src protein tyrosine kinase family. Blk is expressed specifically in the B cell lineage and plays a role in the signal transduction pathway that is restricted to B lymphoid cells (1). Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk (2).

References

  1.  Dymecki, S.M. et al. (1990) Science 247:332.
  2. Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88:7410.

Long Name

B Lymphoid Tyrosine Kinase

Alternate Names

B lymphocyte kinase, B lymphoid tyrosine kinase, BLK nonreceptor tyrosine kinase, EC 2.7.10.2, MGC10442, MODY11, p55-Blk, tyrosine-protein kinase Blk

Entrez Gene IDs

640 (Human); 12124 (Mouse); 364403 (Rat)

Gene Symbol

BLK

UniProt

Additional Blk Products

Product Documents for Recombinant Human Active Blk Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active Blk Protein, CF

For research use only

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