Recombinant Human Active ERK2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1230-KS
Key Product Details
Product Specifications
Source
E. coli-derived human ERK2 protein
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N terminal GST tag
SDS-PAGE
68 kDa
Activity
The specific activity of ERK2 was determined to be 24 nmol/min/mg using a myelin basic protein (MBP) substrate and was equivalent to 639 nmol/min/mg as per radiometric assay.
Scientific Data Images for Recombinant Human Active ERK2 Protein, CF
Recombinant Human Active ERK2 Protein SDS-PAGE
The approximate molecular weight is 68 kDa and the purity is > 80%.Formulation, Preparation and Storage
1230-KS
| Formulation | Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: ERK2
ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).
References
- Boulton, T.G. et al. (1991) Biochemistry 30(1):278.
Long Name
Extracellular Signal-regulated Kinase 2
Alternate Names
ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2
Gene Symbol
MAPK1
UniProt
Additional ERK2 Products
Product Documents for Recombinant Human Active ERK2 Protein, CF
Product Specific Notices for Recombinant Human Active ERK2 Protein, CF
For research use only
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