Recombinant Human Active ITK (aa 352-620) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4587-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human ITK protein
aa 352-620
aa 352-620
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
53 kDa
Activity
The specific activity of ITK was determined to be 35 nmol/min/mg using a myelin basic protein (MBP) substrate.
Scientific Data Images for Recombinant Human Active ITK (aa 352-620) Protein, CF
Recombinant Human Active ITK (aa 352-620) Protein SDS-PAGE.
The approximate molecular weight is 53 kDa and the purity is > 90%.Formulation, Preparation and Storage
4587-KS
| Formulation | Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: ITK
ITK is a member of the TEC family of non-receptor tyrosine kinases. ITK is expressed in T cells and is important for T cell development and activation through the antigen receptor. ITK requires prior activation of Lck, ZAP70, and PI3-kinase for efficient activation and shares major substrates with both Lck and ZAP70 (1). ITK knockout mice show multiple effects on T cell development, cytokine production, and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization, and regulation of the signaling events involved in cytoskeletal reorganization (2).
References
- August, A. et al. (2002) Int. J. Biochem. Cell Biol. 34:1184.
- Finkelstein, L.D. et al. (2004) Trends Cell Biol. 14:443.
Long Name
IL-2-inducible T-cell Kinase
Alternate Names
EMT, LYK, PSCTK2, Tcsk
Gene Symbol
ITK
UniProt
Additional ITK Products
Product Documents for Recombinant Human Active ITK (aa 352-620) Protein, CF
Product Specific Notices for Recombinant Human Active ITK (aa 352-620) Protein, CF
For research use only
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