Recombinant Human Active Lck Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3704-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Lck protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
84 kDa
Activity
The specific activity of Lck is typically 180-244 nmol/min/mg using a Poly (Glu:Tyr, 4:1) synthetic peptide substrate.
Scientific Data Images for Recombinant Human Active Lck Protein, CF
Recombinant Human Active Lck Protein SDS-PAGE.
The approximate molecular weight is 84 kDa and the purity is > 95%.Formulation, Preparation and Storage
3704-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: Lck
Lck (p56lck) is a member of the Src family of non-receptor tyrosine kinases. It was identified as a gene rearranged and over-expressed in the murine lymphoma LSTRA, most likely as a result of the insertion of Moloney murine leukemia virus DNA immediately adjacent to the gene (1). Lck behaves as a proto-oncogene and can lead to cell transformation upon activation. A number of human cancer cell lines show over-expression of Lck, pointing to a possible role for this kinase in the initiation and maintenance of the transformed state in human cancers (2).
References
- Fischer, S. et al. (1987) Biochem. Biophys. Res. Commun. 143:819.
- Veillette, A. et al. (1987) Oncogene Res. 1:357.
Long Name
Lymphocyte Protein Tyrosine Kinase
Alternate Names
Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16
Gene Symbol
LCK
UniProt
Additional Lck Products
Product Documents for Recombinant Human Active Lck Protein, CF
Product Specific Notices for Recombinant Human Active Lck Protein, CF
For research use only
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