Recombinant Human Active MAPKAPK2 (46-end) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3705-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human MAPKAPK2 protein
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
68 kDa
Activity
The specific activity of MAPKAPK2 is typically 233-315 nmol/min/mg using a synthetic peptide substrate (RRLNRQLSVA-amide) (see Activity Assay Protocol).
Scientific Data Images for Recombinant Human Active MAPKAPK2 (46-end) Protein, CF
Recombinant Human Active MAPKAPK2 (46-end) Protein SDS-PAGE.
The approximate molecular weight is 68 kDa and the purity is > 90%.Formulation, Preparation and Storage
3705-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: MAPKAPK2
MAPKAPK2 (MAPKAP kinase 2) is a Ser/Thr protein kinase which is regulated via direct phosphorylation by p38 MAP kinase (1). In conjunction with p38 MAP kinase, MAPKAPK2 is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation, and cell proliferation. Heat shock protein 27 (HSP27) has been shown to be one of the substrates of MAPKAPK2 in vivo.
References
- Stokoe, D. et al. (1993) Biochem. J. 296:843.
Long Name
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2
Alternate Names
MK2, Rps6kc1
Gene Symbol
MAPKAPK2
UniProt
Additional MAPKAPK2 Products
Product Documents for Recombinant Human Active MAPKAPK2 (46-end) Protein, CF
Product Specific Notices for Recombinant Human Active MAPKAPK2 (46-end) Protein, CF
For research use only
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