Recombinant Human Active p38 alpha Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5477-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human p38 alpha protein
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
67 kDa
Activity
The specific activity of p38 alpha is typically 148-201 nmol/min/mg using an ATF2 substrate.
Scientific Data Images for Recombinant Human Active p38 alpha Protein, CF
Recombinant Human Active p38 alpha Protein SDS-PAGE.
The approximate molecular weight is 67 kDa and the purity is > 85%.Formulation, Preparation and Storage
5477-KS
| Formulation | Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 10 mM Glutathione, 0.1 mM EDTA, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: p38 alpha
p38 alpha (SAPK2A) is a member of the p38 MAPK family which are activated by various environmental stresses and pro-inflammatory cytokines (1). The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs) or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase (2). The substrates of p38 include transcription regulator ATF2, MEF2C, MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.
References
- Han, J. et al. (1994) Science 265:808.
- Ge, B. et al. (2002) Science 295:1291.
Alternate Names
MAPK14, SAPK2a
Gene Symbol
MAPK14
UniProt
Additional p38 alpha Products
Product Documents for Recombinant Human Active p38 alpha Protein, CF
Product Specific Notices for Recombinant Human Active p38 alpha Protein, CF
For research use only
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