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Recombinant Human Active p70 S6 Kinase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 896-KS

R&D Systems, part of Bio-Techne
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896-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human p70 S6 Kinase protein

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N‑terminal His tag

SDS-PAGE

76 kDa

Activity

The activity of p70S6K was determined to be 73 nmol/min/mg using a synthetic peptide substrate.

Scientific Data Images for Recombinant Human Active p70 S6 Kinase Protein, CF

Recombinant Human Active p70 S6 Kinase Protein SDS-PAGE

Recombinant Human Active p70 S6 Kinase Protein SDS-PAGE.

The approximate molecular weight is 76 kDa and the average purity is 85%.

Formulation, Preparation and Storage

896-KS
Formulation Supplied in 50 mM sodium phosphate (pH 7.0), 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, and 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ -70°C for up to one year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: p70 S6 Kinase

p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues . p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes in the cell cycle and increases 20-fold in G1 cells released from G0 (2). p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as at T389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK-1) (1, 2).

References

  1. Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
  2. Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.

Alternate Names

p70-alpha, RPS6KB1, S6K1, STK14A

Entrez Gene IDs

6198 (Human); 72508 (Mouse); 83840 (Rat)

Gene Symbol

RPS6KB1

UniProt

Additional p70 S6 Kinase Products

Product Documents for Recombinant Human Active p70 S6 Kinase Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active p70 S6 Kinase Protein, CF

For research use only

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