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Recombinant Human Active PIM1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4588-KS

R&D Systems, part of Bio-Techne
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4588-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PIM1 protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N-terminal GST tag

SDS-PAGE

62 kDa

Activity

The specific activity of PIM1 is typically 269-363 nmol/min/mg using a synthetic peptide substrate.

Scientific Data Images for Recombinant Human Active PIM1 Protein, CF

Recombinant Human Active PIM1 Protein SDS-PAGE

Recombinant Human Active PIM1 Protein SDS-PAGE.

The approximate molecular weight is 62 kDa and the purity is > 90%.

Formulation, Preparation and Storage

4588-KS
Formulation Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: PIM1

PIM1 is a proto-oncogene that belongs to a family of Ser/Thr protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T cell lymphomas in mice, PIM1 is involved in the control of cytokine-mediated cell proliferation, differentiation, and survival of lymphoid and myeloid cells as well as others (1). Expression of PIM1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational, and post-translational (2). Expression of PIM1 is mediated through activation of the JAK/STAT pathway.

References

  1. Meeker, T.C. et al. (1987) J. Cell. Biochem. 35:105.
  2. Friedmann, M. et al. (1992) Arch. Biochem. Biophys. 298:594.

Long Name

Proviral Integration Site 1

Alternate Names

EC 2.7.11.1, Oncogene PIM1, PIM, pim-1 kinase 44 kDa isoform, pim-1 oncogene, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1

Entrez Gene IDs

5292 (Human); 18712 (Mouse); 24649 (Rat)

Gene Symbol

PIM1

Additional PIM1 Products

Product Documents for Recombinant Human Active PIM1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active PIM1 Protein, CF

For research use only

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