Recombinant Human Active PKA C alpha Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4268-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PKA C alpha protein
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
69 kDa
Activity
The activity of PKA C alpha is typically 1626-2201 nmol/min/mg using a synthetic peptide substrate.
Reviewed Applications
Read 3 reviews rated 4.7 using 4268-KS in the following applications:
Scientific Data Images for Recombinant Human Active PKA C alpha Protein, CF
Recombinant Human Active PKA C alpha Protein SDS-PAGE.
The approximate molecular weight is 69 kDa and the purity is > 90%.Formulation, Preparation and Storage
4268-KS
| Formulation | Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 10 mM Glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: PKA C alpha
The catalytic subunit C-alpha of PKA (PKA C alpha) is a member of the Ser/Thr protein kinase family and has been assigned to chromosome region 19p13.1 (1). Null mutation in PKA C alpha leads to early postnatal lethality in the majority of C-alpha knockout mice. Surprisingly, a small percentage of C-alpha knockout mice, although runted, survived to adulthood. In these animals, compensatory increases in C-beta levels occurred in brain whereas many tissues, including skeletal muscle, heart, and sperm contained less than 10% of the normal PKA activity (2).
References
- Tasken, K. et al. (1996) Genomics 36:535.
- Skalhegg, B.S. et al. (2002) Molec. Endocr. 16:630.
Long Name
cAMP-dependent Protein Kinase Catalytic Subunit alpha
Alternate Names
Cs, Pkaca, PKCA1, PKCD, PRKACA
Gene Symbol
PRKACA
UniProt
Additional PKA C alpha Products
Product Documents for Recombinant Human Active PKA C alpha Protein, CF
Product Specific Notices for Recombinant Human Active PKA C alpha Protein, CF
For research use only
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