Recombinant Human Active PLK1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3804-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PLK1 protein
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal His tag
SDS-PAGE
70 kDa
Activity
The specific activity of PLK1 was equivalent to 42 nmol/min/mg as per Activity Assay Protocol and was equivalent to 17 nmol/min/mg as per radiometric assay.
Scientific Data Images for Recombinant Human Active PLK1 Protein, CF
Recombinant Human Active PLK1 Protein SDS-PAGE.
The approximate molecular weight is 70 kDa and the purity is > 90%.Formulation, Preparation and Storage
3804-KS
| Formulation | Supplied in 50 mM Sodium Phosphate (pH 7.0), 300 mM NaCl, 150 mM Imidazole, 0.1 mM PMSF, 0.25 mM DTT, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: PLK1
PLK1 is a member of the Polo-Like Kinase family that localizes to centrosomes or spindle pole bodies and undergoes dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics (1). PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death (2).
References
- Nigg, E.A. et al. (1996) Exp. Cell Res. 229:174.
- Dai, W. et al. (2003) Prog. Cell Cycle Res. 5:327.
Long Name
Polo-like Kinase 1
Alternate Names
STPK13
Gene Symbol
PLK1
UniProt
Additional PLK1 Products
Product Documents for Recombinant Human Active PLK1 Protein, CF
Product Specific Notices for Recombinant Human Active PLK1 Protein, CF
For research use only
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