Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF
R&D Systems, part of Bio-Techne | Catalog # 4540-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Raf-1 protein
aa 306-648
aa 306-648
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
63 kDa
Activity
The specific activity of Raf-1 is typically 5100-6900 nmol/min/mg using a myelin basic protein (MBP) substrate (see Activity Assay Protocol).
Scientific Data Images for Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF
Recombinant Human Active Raf-1 (Y340E Y341E, 306-end) SDS-PAGE.
The approximate molecular weight is 63 kDa and the purity is > 85%.Formulation, Preparation and Storage
4540-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM Glutathione, 0.1 mM EDTA, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: Raf-1
Raf-1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated Raf-1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the Serine/Threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation, and cell migration (2).
References
- Rapp, U. et al. (1983) Proc. Natl. Acad. Sci. USA 80:4218.
- Li, P. et al. (1991) Cell 64:479.
Long Name
v-raf-1Murine Leukemia Viral Oncogene Homolog 1
Alternate Names
c-Raf-1, Raf1, v-Raf-1
Gene Symbol
RAF1
UniProt
Additional Raf-1 Products
Product Documents for Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF
Product Specific Notices for Recombinant Human Active Raf-1 (Y340E Y341E, 306-end), CF
For research use only
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