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Recombinant Human Active RSK1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 992-KS

R&D Systems, part of Bio-Techne
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992-KS-010

Key Product Details

Source

Sf 9 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 9 (baculovirus)-derived human RSK1 protein

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Using an N terminal GST Tag

SDS-PAGE

108 kDa

Activity

The specific activity of RSK1 was determined to be 210 nmol/min/mg using a synthetic peptide substrate (see Activity Assay Protocol).

Scientific Data Images for Recombinant Human Active RSK1 Protein, CF

Recombinant Human Active RSK1 Protein SDS-PAGE

Recombinant Human Active RSK1 Protein SDS-PAGE

The approximate molecular weight is 108 kDa and the purity is > 90%.

Formulation, Preparation and Storage

992-KS
Formulation Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70°C for up to one year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: RSK1

RSK1 is a member of the RSK (ribosomal S6 kinase) family that consists of growth factor-regulated serine/threonine kinases. RSK1 contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. RSK1 encodes a predicted 735 amino acid protein containing 2 distinct consensus ATP-binding site sequences. RSK1 transcript is present in lymphocytes, skeletal muscle, liver, and adipose tissue (1). RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation (2).

References

  1. Moller, D.E. et al. (1994) Am. J. Physiol. 266:C351.
  2. Gross, S.D. et al. (1999) Science 286:1365.

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Entrez Gene IDs

6195 (Human); 20111 (Mouse); 81771 (Rat)

Gene Symbol

RPS6KA1

Additional RSK1 Products

Product Documents for Recombinant Human Active RSK1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Active RSK1 Protein, CF

For research use only

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