Recombinant Human Active SGK1 (60-end) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3200-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human SGK1 protein
aa 60-431
With an N-terminal GST tag
aa 60-431
With an N-terminal GST tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
SDS-PAGE
73 kDa
Activity
The specific activity of SGK1 was determined to be 99 nmol/min/mg using a Akt (PKB) peptide substrate as per activity assay protocol.
Scientific Data Images for Recombinant Human Active SGK1 (60-end) Protein, CF
Recombinant Human Active SGK1 (60-end) Protein SDS-PAGE.
The approximate molecular weight is 73 kDa and the purity is > 90%.Formulation, Preparation and Storage
3200-KS
| Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: SGK1
SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK 1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1 mRNA is expressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1 (2). SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1.
References
- Kobayashii, T. et al. (1999) Biochem. J. 1:189.
- Brunet, A. et al. (2001) Mol. Cell. Biol. 21:952.
Long Name
Serum/Glucocorticoid Regulated Kinase 1
Alternate Names
SGK
Gene Symbol
SGK1
UniProt
Additional SGK1 Products
Product Documents for Recombinant Human Active SGK1 (60-end) Protein, CF
Product Specific Notices for Recombinant Human Active SGK1 (60-end) Protein, CF
For research use only
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