Recombinant Human Active SYK Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4594-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human SYK protein
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
100 kDa
Activity
The specific activity of SYK is typically 79-107 nmol/min/mg using a poly (Glu:Tyr, 4:1) synthetic peptide substrate.
Scientific Data Images for Recombinant Human Active SYK Protein, CF
Recombinant Human Active SYK Protein SDS-PAGE.
The approximate molecular weight is 100 kDa and the purity is > 85%.Formulation, Preparation and Storage
4594-KS
| Formulation | Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 10 mM Glutathione, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: SYK
SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells. It is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. In B cells, SYK plays a crucial role in intracellular signal transduction induced by oxidative stress as well as antigen receptor engagement (1). SYK has been shown to act as a potential tumor suppressor in breast cancer. Absence of SYK protein in primary breast tumors is correlated with poor outcomes. SYK deficient cells have increased motility that is restored to normalcy by replacement with wild-type SYK (2).
References
- Takano, T. et al. (2002) Antioxid. Redox. Signal. 4:533.
- Navara, C.S. et al. (2004) Curr. Pharm. Des. 10:1739.
Long Name
Spleen Tyrosine Kinase
Alternate Names
DKFZp313N1010, EC 2.7.10, EC 2.7.10.2, FLJ25043, spleen tyrosine kinaseFLJ37489, tyrosine-protein kinase SYK
Gene Symbol
SYK
UniProt
Additional SYK Products
Product Documents for Recombinant Human Active SYK Protein, CF
Product Specific Notices for Recombinant Human Active SYK Protein, CF
For research use only
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