Recombinant Human Activin RIIA Fc Chimera Protein

R&D Systems, part of Bio-Techne | Catalog # 340-R2

R&D Systems, part of Bio-Techne
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Carrier Free
340-R2-100/CF
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With Carrier
340-R2-100
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P27037

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Activin RIIA Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Activin RIIA protein
Human Activin RIIA
(Ser25 - Pro134)
Accession # P27037		 IEGRMD Human IgG1
(Pro100 - Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser25

Predicted Molecular Mass

40 kDa (monomer)

SDS-PAGE

50 kDa, reducing conditions

Activity

Measured by its ability to inhibit Activin A-induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
Approximately 0.03-0.1 µg/mL of rhActivin RIIA/Fc Chimera will inhibit 50% of the biological response due to 3 ng/mL of rhActivin A.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 340-R2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 340-R2/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIIA

Activin proteins are involved in a wide range of biological processes including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, the regulation of reproductive physiology, inflammation, and carcinogenesis (1 ‑ 3). They function through heteromeric complexes of type I and type II serine/threonine kinase receptors (2, 4). Dimeric ligands bind to a type II receptor, such as Activin Receptor IIA (ActRIIA), which then associates with a type I receptor to initiate signal transduction (4). ActRIIA mediates the pleiotropic effects of Activins and Inhibins as well as several members of the BMP and GDF families of TGF-beta like proteins (4). Mature human ActRIIA is a 70 kDa glycoprotein that consists of a 116 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 352 aa cytoplasmic region that includes the kinase domain and a PDZ-binding motif (5). Within the ECD, human ActRIIA shares 98% aa sequence identity with mouse and rat ActRIIA. Signaling through ActRIIA is modulated by its interaction with RGM-B/DRAGON, Cripto, Endoglin/CD105, TGF-beta RIII/Betaglycan, or BAMBI (6 - 10). These interactions can enhance ligand-induced signaling or interfere with signaling by preventing ActRIIA association with type I receptors (6 - 9). Activin-induced responses can also be limited by the enhanced internalization of ActRIIA following its association with the cytoplasmic proteins ARIP1 and ARIP2 (11, 12).

References

  1. Welt, C. et al. (2002) Exp. Biol. Med. 227:724.
  2. Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
  3. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
  4. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  5. Matzuk, M.M. and A. Bradley (1992) Biochim. Biophys Acta 1130:105.
  6. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
  7. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
  8. Lewis, K.A. et al. (2000) Nature 404:411.
  9. Onichtchouk, D. et al. (1999) Nature 401:480.
  10. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  11. Shoji, H. et al. (2000) J. Biol. Chem. 275:5485.
  12. Matsuzaki, T. et al. (2002) J. Biol. Chem. 277:19008.

    Long Name

    Activin Receptor IIA

    Alternate Names

    ActivinRIIA, ACVR2A, AVR2A

    Entrez Gene IDs

    92 (Human); 11480 (Mouse)

    Gene Symbol

    ACVR2A

    UniProt

    P27037

    Additional Activin RIIA Products

    Product Documents for Recombinant Human Activin RIIA Fc Chimera Protein

    Product Datasheets

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Download SDS 340-R2 (with carrier)
    Download SDS 340-R2/CF (carrier free)

    Product Specific Notices for Recombinant Human Activin RIIA Fc Chimera Protein

    For research use only

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