Recombinant Human ADAM28 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9325-AD

R&D Systems, part of Bio-Techne
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9325-AD-050
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Key Product Details

Source

CHO

Accession #

Q9UKQ2

Conjugate

Unconjugated

Applications

Bioactivity

View all ADAM28 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ADAM28 protein
Leu72 & Leu191-Ala623, with a C-terminal 6-His tag

Purity

>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu72 & Leu191

Predicted Molecular Mass

49 & 62 kDa

SDS-PAGE

59-80 kDa, reducing conditions

Activity

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
The ED50 for this effect is 0.250-2.50 μg/mL.

Reviewed Applications

Read 2 reviews rated 5 using 9325-AD in the following applications:

Scientific Data Images for Recombinant Human ADAM28 Protein, CF

Recombinant Human ADAM28 Protein Bioactivity

Recombinant Human ADAM28 Protein Bioactivity.

Recombinant Human ADAM28 (Catalog # 9325-AD) induces adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.250-2.50 μg/mL.

Formulation, Preparation and Storage

9325-AD
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 200 μg/mL in water.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM28

A distintegrin and metalloprotease domain-containing protein 28 (ADAM28), also known as MDC-L, is a member of the M12B peptidase family of enzymes. It is synthesized as an approximately 80-90 kDa glycosylated proprotein that is processed to a mature form later in the secretory pathway (1-3). After the removal of the propeptide that contains a cysteine switch motif, the activated form of ADAM28 consists of a 467 amino acid (aa) extracellular domain (ECD) which contains a peptidase, disintegrin, cysteine-rich and EGF-like domains, followed by a 21 aa transmembrane segment and an 89 aa cytoplasmic domain (3). Alternative splicing generates additional isoforms with a variety of substitutions and deletions in the cysteine-rich and EGF-like domains (1). Within the ECD, human ADAM28 shares 73% aa sequence identity with mouse and rat ADAM28 (3).  ADAM28 is a cell surface protein that is involved in a variety of cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis (4-6), and exhibits catalytic activity to insulin-like growth factor binding protein-3 (7). Like many members of the ADAM proteins, the disintegrin domain of ADAM28 interacts with integrin to influence cell adhesion and cell-cell interaction (8). ADAM28 can mediate the adhesion of the Jurkat cells, T-lymphoma cell line, through integrin alpha4b1(9). 

References

  1. Roberts, C.M. et al. (1999) J. Biol. Chem. 274:29251.
  2. McGinn, O.J. et al. (2011) Cell Biol. Int. 35:1043.
  3. Howard, L. et al. (2000) Biochem. J. 348:21.
  4. Mochizuki, S. and Okada Y, (2007) Cancer Sci. 98:621.
  5. Schlondorff, J. et al. (1999) J. Cell Sci. 112:3603.
  6. Blobel, C.P. (1997) Cell 90:589.
  7. Mochizuki S. and Okada Y. (2009) Curr. Pharm. Des. 15:2349.
  8. Edwards, L. et al. (2008) Molecular Aspects of Medicine 29:258.
  9. Bridges, L. et al. (2002) J. Biol. Chem. 277:3784.

Long Name

A Disintegrin and Metalloproteinase Domain 28

Alternate Names

Dtgn1, eMDCII, MDC-Lm, MDC-Ls, MDCL, Protein-L

Entrez Gene IDs

10863 (Human); 13522 (Mouse); 290344 (Rat)

Gene Symbol

ADAM28

UniProt

Q9UKQ2

Additional ADAM28 Products

Product Documents for Recombinant Human ADAM28 Protein, CF

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Product Specific Notices for Recombinant Human ADAM28 Protein, CF

For research use only

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