Recombinant Human ADAM9 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 939-AD

R&D Systems, part of Bio-Techne
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939-AD-020
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Key Product Details

Source

NS0

Accession #

Q13443

Structure / Form

Mature form

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ADAM9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ADAM9 protein
Ala206-Asp697, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala206

Predicted Molecular Mass

55 kDa

SDS-PAGE

64 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003).
The specific activity is >1.0 pmol/min/µg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 939-AD in the following applications:

Formulation, Preparation and Storage

939-AD
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ADAM9

ADAM9, also known as MDC9 or meltrin gamma, is a member of the ADAM family that contains a disintegrin and metalloprotease‑like domain (1). Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc‑binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha), the p75 TNF receptor, the beta-amyloid protein precursor, and the c‑kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane‑anchored heparin-binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin beta-chain and fibronectin (2,4). Besides its catalytic activity, ADAM9 functions as an adhesion molecule through binding of its disintegrin domain to integrins such as alphav beta5 and alpha6 beta1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3
(SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

References

  1. Moss, et al. (2001) DDT 6:417.
  2. Roghan, et al. (1999) J. Biol. Chem. 274:3531.
  3. Izumi, et al. (1998) EMBO J. 17:7260.
  4. Schwettmann and Tschesche (2001) Prot. Expre. & Purif. 21:65.
  5. Nath, et al. (2000) J. Cell Sci. 113:2319.
  6. Zhou, et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
  7. Howard, et al. (1999) J. Biol. Chem. 274:31693.
  8. Weskamp, et al. (1996) J. Cell. Biol. 132:717.

Long Name

A Disintegrin and Metalloprotease-like Domain 9

Alternate Names

MCMP, MDC9, Meltrin gamma

Entrez Gene IDs

8754 (Human); 11502 (Mouse)

Gene Symbol

ADAM9

UniProt

Q13443

Additional ADAM9 Products

Product Documents for Recombinant Human ADAM9 Protein, CF

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Product Specific Notices for Recombinant Human ADAM9 Protein, CF

For research use only

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