Recombinant Human ADAMTS15 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5149-AD

R&D Systems, part of Bio-Techne
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5149-AD-020
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Key Product Details

Source

CHO

Accession #

Q8TE58

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ADAMTS15 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ADAMTS15 protein
Gly18-Cys682 (pro) & Phe213-Cys682 (mature), both with a C-terminal 10-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly18 & Phe213

Predicted Molecular Mass

74 kDa (pro), 53 kDa (mature)

SDS-PAGE

85 kDa and 65 kDa, reducing conditions

Activity

Measured by its ability to cleave Recombinant Human Aggrecan G1-IGD-G2 Domains (Catalog # 1220-PG).
Cleavage of 1 µg of rhAggrecan is >90%, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 4 using 5149-AD in the following applications:

Formulation, Preparation and Storage

5149-AD
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ADAMTS15

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) is a group of nineteen secreted zinc binding metalloproteases that is involved in development, inflammation, angiogenesis and several pathological conditions (1, 2). They are synthesized as zymogens which have a pro-domain that is removed by furin-like protein convertases. The mature secreted enzymes consist of catalytic, disintegrin-like, type I thrombospondin (TSP) motif, cysteine-rich, and spacer domains followed by zero to fourteen additional TSP motifs. Among ADAMTS proteases, ADAMTS1, -4, -5, -8, and -15 form a subfamily that possess aggrecanase activity (1-3). While ADAMTS4 and -5 (also known as aggrecanse-1 and -2, respectively) (4) have been well studied, the enzymatic activity of ADAMTS15 remains to be explored. It is suggested that ADAMTS15 may be involved in aggrecan degradation and breast cancer (5, 6). ADAMTS15 may also exhibit tumor suppressor activities since it contains genetic mutations in human colorectal carcinomas and restrains tumor growth and invasion in both in vitro and in vivo studies (7). The purified rhADAMTS15 is truncated and ends before the spacer domain.

References

  1. Porter, S. et al. (2005) Biochem. J. 386:15.
  2. Jones, G.C. and G.P. Riley (2005) Arthritis Res. Ther. 7:160.
  3. Nicholson, A.C. et al. (2005) BMC Evol. Biol. 5:11.
  4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  5. Porter, S. et al. (2006) Intl. J. Cancer 118:1241.
  6. Kevorkian, L. et al. (2004) Arthritis Rheum. 50:131.
  7. Viloria, C.G. et al. (2009) Cancer Res. 69:4926.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 15

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, preproprotein, ADAM metallopeptidase with thrombospondin type 1 motif, 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15, EC 3.4.24, EC 3.4.24.-, EC 3.4.24.14, EC 3.4.24.82, metalloprotease disintegrin 15 with thrombospondin domains, MGC126403

Entrez Gene IDs

170689 (Human); 235130 (Mouse); 300474 (Rat)

Gene Symbol

ADAMTS15

UniProt

Q8TE58

Additional ADAMTS15 Products

Product Documents for Recombinant Human ADAMTS15 Protein, CF

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Product Specific Notices for Recombinant Human ADAMTS15 Protein, CF

For research use only

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