Recombinant Human Adenosine Deaminase/ADA Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7048-AD

R&D Systems, part of Bio-Techne
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7048-AD-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P00813

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Adenosine Deaminase/ADA Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Adenosine Deaminase/ADA protein
Met1-Leu363, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments

Predicted Molecular Mass

42 kDa

SDS-PAGE

40-42 kDa, reducing conditions

Activity

Measured by the ability to catalyze the hydrolytic deamination of adenosine to inosine.
The specific activity is >44,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7048-AD
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Adenosine Deaminase/ADA

Adenosine Deaminase (ADA, adenosine aminohydrolase) is one of the key enzymes of purine nucleotide catabolism. It catalyses the hydrolytic deamination of adenosine and deoxy‑adenosine to inosine and deoxyinosine (1, 2). ADA is expressed in virtually all tissues and is expressed at high levels in T-lymphocytes. Adenosine Deaminase deficiency can cause a form of SCID (severe combined immunodeficiency) and lymphopenia in both B- and T-cell lineages (3, 4). ADA can be used as a sensitive diagnostic marker for tuberculous pleuritis (5). Although it is primarily a cytosolic enzyme, ADA is known to be a positive regulator of T-cell co-activation due to its binding to CD26 at the cell surface. The interaction of ADA with CD26 regulates lymphocyte-epithelial cell adhesion (6).

References

  1. Wolfenden, R.V. et al. (1969) Biochemistry 6:2412.
  2. Lowenstein, J.M. (1972) Physiol. Rev. 52:382.
  3. Giblett, E.R. et al. (1972) Lancet 2:1067.
  4. Coleman, M.S. et al. (1978) J. Biol. Chem. 253:1619.
  5. Baba, K. et al. (2008) PLoS ONE 3:e2788.
  6. Gines, S. et al. (2002) Biochem. J. 361:203.

Long Name

Adenosine Aminohydrolase

Alternate Names

ADA, ADA1

Entrez Gene IDs

100 (Human); 11486 (Mouse); 24165 (Rat)

Gene Symbol

ADA

UniProt

P00813

Additional Adenosine Deaminase/ADA Products

Product Documents for Recombinant Human Adenosine Deaminase/ADA Protein, CF

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Product Specific Notices for Recombinant Human Adenosine Deaminase/ADA Protein, CF

For research use only

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