Recombinant Human Adenosine Kinase/ADK Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8024-AK

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
8024-AK-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

P55263

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ADK Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human ADK protein
Ala2-His362 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

41 kDa

SDS-PAGE

43-45 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate Adenosine.
The specific activity is >7 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8024-AK
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ADK

Adenosine kinase (ADK) converts adenosine into 5'-adenosine-monophosphate. ADK is therefore key in regulating the concentration of adenosine, an essential homeostatic and metabolic regulator in all living systems (1). Adenosine not only activates specific signaling pathways by binding to adenosine receptors but also is a primordial metabolite and regulator of numerous biochemical reactions that are related to metabolism and genetics. ADK dysfunction is involved in several pathologies, including diabetes, epilepsy, and cancer. For example, inhibiting ADK activity promotes the replication of primary insulin-producing beta cells in diabetic mouse, rat, and pig (2); mutation of ADK causes hypermethioninemia, a condition with an excess of methionine in the blood (3); ADK is also identified as a neuropathological marker of the epileptic brain (4). Given its significant roles in various diseases, ADK is a rational therapeutic target (1). ADK is widely expressed in all tissues, with elevated levels in placenta, liver, muscle and kidney (5). The enzymatic activity of recombinant human ADK is measured using a phosphatase-coupled method (6).

References

  1. Boison, D. (2013) Pharmacol. Rev. 65:906.
  2. Annes, J.P. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:3915.
  3. Bjursell, M.K. et al. (2011) Am. J. Hum. Genet. 89:507.
  4. Aronica, E. et al. (2013) Neurochem. Int. in press.
  5. Spychala, J. et al. (1996) Proc. Natl. Acad. Sci. U.S.A. 93:1232.
  6. Wu, Z.L. (2011) PLoS One 6:e23172.

Long Name

Adenosine Kinase

Alternate Names

AK

Entrez Gene IDs

132 (Human); 11534 (Mouse); 25368 (Rat)

Gene Symbol

ADK

UniProt

P55263

Additional ADK Products

Product Documents for Recombinant Human Adenosine Kinase/ADK Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Adenosine Kinase/ADK Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.