Recombinant Human ADP-sugar Pyrophosphatase/NUDT5, CF
R&D Systems, part of Bio-Techne | Catalog # 6414-NH
Key Product Details
Product Specifications
Source
E. coli-derived human ADP-Sugar Pyrophosphatase/NUDT5 protein
Glu2-Phe219, with an N-terminal Met and 6-His tag
Glu2-Phe219, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
25 kDa
SDS-PAGE
32-37 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze ADP-ribose to AMP and ribose-5-phosphate.
The specific activity is >1,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >1,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6414-NH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: ADP-Sugar Pyrophosphatase/NUDT5
ADP-Sugar Pyrophosphatase (NUDT5) is a member of the nudix superfamily of enzymes. Members of this family are pyrophosphohydrolases that act upon substrates with the general structure of a nucleoside (Nu) diphosphate (di) linked to another moiety, X (NDP-X) to yield NMP plus P-X (1). Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3). NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP-ribosylation at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucleosides (5, 6).
References
- McLennan, A.G. (2006) Cell Mol. Life Sci. 63:123.
- Zha, M. et al. (2006) J. Mol. Biol. 364:1021.
- Zha, M. et al. (2008) J. Mol. Biol. 379:568.
- Yu, H.N. et al. (2007) Biochem. Biophys. Res. Comm. 354:764.
- Hori, M. et al. (2010) Free Radic. Biol. Med. 48:1197.
- Kamiya, H. et al. (2009) DNA Repair (Amst) 8:1250.
Alternate Names
Nudix motif 5, YSA1, YSA1H
Entrez Gene IDs
11164 (Human)
Gene Symbol
NUDT5
UniProt
Additional ADP-Sugar Pyrophosphatase/NUDT5 Products
Product Documents for Recombinant Human ADP-sugar Pyrophosphatase/NUDT5, CF
Product Specific Notices for Recombinant Human ADP-sugar Pyrophosphatase/NUDT5, CF
For research use only
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