Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7678-DH

R&D Systems, part of Bio-Techne
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7678-DH-020
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Key Product Details

Source

E. coli

Accession #

P42330

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Aldo-keto Reductase 1C3/AKR1C3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Aldo-keto Reductase 1C3/AKR1C3 protein
Asp2-Tyr323, with an N-terminal Met and 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

38 kDa

SDS-PAGE

36-37 kDa, reducing conditions

Activity

Measured by its ability to catalyze the reduction of phenanthrenequinone.
The specific activity is >115 pmol/min/μg, as measured under the described conditions.

Reviewed Applications

Read 2 reviews rated 5 using 7678-DH in the following applications:

Formulation, Preparation and Storage

7678-DH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Aldo-keto Reductase 1C3/AKR1C3

AKR1C3 is a member of the aldo-keto reductase (AKR) superfamily. It catalyzes oxidation/reduction reactions at the 3-alpha, 20-alpha, and 17-beta positions of steroids (1). It is also known as Prostaglandin F synthase as it reduces prostaglandin D2 to F2, and therefore may play a role in allergic conditions such as asthma (2). Elevated expression of AKR1C3 in endometrium that results in enhanced estrogen action may lead to endometrial cancer (3). It is also up‑regulated in squamous cell carcinoma of head and neck (4). AKR1C3 is found to be a novel suppressor of cell differentiation that provides a plausible target for the non-cyclooxygenase-dependent antineoplastic actions of nonsteroidal anti-inflammatory drugs (5).

References

  1. Penning, T. M. et al. (2000) Biochem J. 351:67.
  2. Suzuki-Yamamoto, T. et al. (1999) FEBS Lett. 462:335.
  3. Rizner, T. L. et al. (2006) Mol. Cell. Endocrinol. 248:126.
  4. Li, S. et al. (2004) Br. J. Cancer 90:1093.
  5. Desmond, J. C. et al. (2003) Cancer Res. 63:505.

Long Name

Aldo-keto Reductase Family 1, Member C3

Alternate Names

AKR1C3, AldoketoReductase 1C3, DD3, DDX, HA1753, HAKRB, HSD17B5, PGFS

Entrez Gene IDs

8644 (Human); 105349 (Mouse); 171516 (Rat)

Gene Symbol

AKR1C3

UniProt

P42330 (Human)

Additional Aldo-keto Reductase 1C3/AKR1C3 Products

Product Documents for Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Aldo-keto Reductase 1C3/AKR1C3 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

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